Fig. 2: Co-expression of two N6-MTases leads to increased m6dA levels in the cells. | Communications Biology

Fig. 2: Co-expression of two N6-MTases leads to increased m6dA levels in the cells.

From: Genome-wide deposition of 6-methyladenine in human DNA reduces the viability of HEK293 cells and directly influences gene expression

Fig. 2

a Schematic depiction of the experimental workflow used for the generation of stable double cell lines and the competitive proliferation assay displayed in panel (c). b Quantification of genome-wide m6dA levels relative to the sum of m6dA and dA in parts per million (ppm) by HPLC-MS/MS measurement. Levels were determined for genomic samples with and without expression of different N6-MTases. c Flow cytometry results of the competitive proliferation assay for the cell lines co-expressing two N6-MTases (GFP+/dsRed+) versus non-fluorescent HEK293 cells. The fraction of GFP+/dsRed+ cells was calculated and normalized to day 5 of dox treatment.

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