Fig. 6: m6dA-dependent downregulation of genes is potentially linked to loss of JUN TF binding. | Communications Biology

Fig. 6: m6dA-dependent downregulation of genes is potentially linked to loss of JUN TF binding.

From: Genome-wide deposition of 6-methyladenine in human DNA reduces the viability of HEK293 cells and directly influences gene expression

Fig. 6

a Position weight matrices (PWM) of candidate TFs containing the CcrM target motif. PWMs were obtained from the JASPAR 2022 database (https://jaspar.genereg.net/). b Crystal structure of the Jun/Fos heterodimer (pdb: 1FOS) binding to DNA (orange). Deoxyadenine residues in the GANTC sequence on both strands of the model (red) were modified to m6dA. The distance between the amino group of R279 of JUN and the adjacent m6dA methyl group is indicated. c Flow cytometry results, showing the median GFP expression in cells containing different variants of the EMILIN2 promoter upstream of GFP: unaltered EMILIN2 promoter, A-to-G mutation in the putative JUN (TGANTCA) motif (mut1), or A-to-G mutation in a GANTC motif (mut2) lacking the surrounding T/A bases. d Flow cytometry analysis showing the changes of GFP expression for the different EMILIN2 promoter variants after CcrM induction. Ratios of the median GFP signals for CcrM and CcrM D39A were calculated.

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