Fig. 1: TRP channels and ANOs expression in NHEKs.
From: TRPV3-ANO1 interaction positively regulates wound healing in keratinocytes
a RT-PCR of TRPs and ANOs in NHEKs. Uncropped gel images are shown in Suppl. Fig. 11. b Western blot of ANO1 in NHEKs. The predicted band size of ANO1 is 114 kDa. c, d Calcium imaging in NHEKs. Cam Camphor, a TRPV3 agonist; GSK GSK1016790A, a TRPV4 agonist; Ca2+ (−) Calcium-free bath solution. e Comparison of current–voltage relationships. The red line indicates current–voltage relationship of basal current in NHEKs using a standard bath and pipette solution. The blue line indicates current–voltage relationship in TRPV6-expressing HEK293T cells using a standard bath solution and NMDG-Cl pipette solution. The holding potential was −60 mV and ramp-pulses were applied from −100 to +100 mV for 300 ms duration every 5 s.
