Fig. 2: TRPV3 and ANO1 interaction in HEK293T cells.

a A representative trace of the camphor-induced currents in HEK293T cells expressing both hTRPV3 and hANO1, hANO1 alone or hTRPV3 alone. All data were collected using an NMDG-Cl bath and pipette solutions. Free calcium in the pipette solution was 100 nM. The holding potential was −60 mV and ramp-pulses were applied from −100 to +100 mV for 300 ms duration every 5 s. b Comparison of peak currents of (A) at −60 mV (means ± SEM, N = 6). Statistical significance was determined with a Mann–Whitney test. c Immunoprecipitation of ANO1 or TRPV3 and Western blot of TRPV3 in HEK293T cells transfected with TRPV3 and ANO1 cDNAs, Ano1 alone, TRPV4 alone or pcDNA3.1. Uncropped gel images are shown in Suppl. Fig. 11.