Fig. 3: TRPV3 and ANO1 interaction in NHEKs.

a, b Representative traces of camphor-induced currents in NHEKs using an NMDG-Cl bath solution containing 148 mM chloride (a) or an NMDG-aspartate bath solution containing 4 mM chloride (b). The pipette solution contained 140 mM NMDG-Cl and 100 nM free calcium. The holding potential was −60 mV and ramp-pulses were applied from −100 to +100 mV for 300 ms duration every 5 s. c Comparison of current–voltage relationships of the currents at arrowheads in (a, b). d Calcium imaging of NHEKs upon camphor application with and without (Ca²⁺ (−)) extracellular calcium. e Comparison of camphor-induced peak currents at −60 mV in an NMDG-Cl bath solution with 2 mM (Ca²⁺ (+)) or without (Ca²⁺ (−)) extracellular calcium (means ± SEM, N = 5). Statistical significance was determined with a Mann–Whitney test. f Camphor-induced current with an ANO1 inhibitor, Ani9, using an NMDG-Cl bath solution containing 148 mM chloride.