Fig. 4: TRPV3 and ANO1 interaction in mouse primary skin keratinocytes.

a, b Representative traces of 10 mM camphor-induced currents in primary keratinocytes isolated from WT (a) and TRPV3^−/− (b) mice. All the recordings were performed with standard NMDG-Cl bath and pipette solutions (with 100 nM free calcium in the pipette). The holding potential was −60 mV and ramp-pulses were applied from −100 to +100 mV for 300 ms duration every 3 s. c Comparison of current–voltage relationships. N equals 5 for WT (black) and 4 for TRPV3^−/− (gray) keratinocytes. Error bars indicate SEM. d Comparison of densities of the camphor-induced currents in WT (black) and TRPV3^−/− (gray) keratinocytes at +60 and −60 mV (means ± SEM). Statistical significance was determined with a Mann–Whitney test. e A representative trace of 10 mM camphor-induced currents with Ani9 inhibition in a WT keratinocyte. A representative trace of 10 mM camphor-induced currents with Ani9 inhibition in a WT keratinocyte. The holding potential was −60 mV and ramp-pulses were applied from −100 to +100 mV for 300 ms duration every 3 s.