Fig. 7: Expression of cation-chloride cotransporter genes and calculated chloride concentrations in NHEKs.
From: TRPV3-ANO1 interaction positively regulates wound healing in keratinocytes
a RT-PCR assessment of cation-chloride cotransporter genes in NHEKs. RT indicates reverse transcription. Uncropped gel images are shown in Suppl. Fig. 11. b A representative image of chloride ion-quenched fluorescent indicator, MQAE, in NHEKs. The scale bar indicates 10 μm. c A calibration curve and calculated intracellular chloride concentrations in NHEKs. The calibration curve was made with a Stern–Volmer plot, F0/F = 1 + Kq[Cl]. F0: fluorescence intensity at 0 mM chloride, F fluorescence intensity at each chloride concentration, Kq extinction coefficient.
