Fig. 3: The focusing ability under different applications.

a Handling of the experimental mouse and the schematic of the focusing position. b The focus PBR right after the focusing procedure finished at several positions on the mouse ear. c Images of the conventional and fCOAT generated focus profile through living mouse ear at two different positions shown in a at different time points. Scale bar, 20 μm. d The intensity profiles of the peak position of the focus shown in c as a function of time. e Schematics of the generation of small (top) and large (bottom) focus. Scale bar, 20 μm. f Images of the conventional and fCOAT generated focus profile through brain tissue slices (100, 200, 300, 400, and 500 μm thick). The intensity profile of the dotted section line in the figures are shown at the bottom edge of the images. Scale bar, 20 μm. g A comparison of the intensity profiles between small and large focus through 200 μm brain slices. h The statistical data of the FWHM focal spot sizes and the PBR of the focus as the functions of tissue thickness. The shadow represents the centralized area of FWHM data points. Error bars represent the SE of sixteen measurements taken at different locations.