Fig. 4: Gene expression heterogeneity of neutrophils.
From: A vein wall cell atlas of murine venous thrombosis determined by single-cell RNA sequencing
a Immunostaining of neutrophil marker Ly6G within IVC/thrombus cross-sections 48 h after IVC ligation (left panel). DAPI was used to stain nuclei. Thrombus depicted with asterisk. Vein wall/thrombus interface highlighted by white dotted line. Area encompassed by white box shown magnified in right panel. N = 3 mice for each group. b UMAP plot of sub-populations in neutrophils of sham and DVT data combined. c Relative distribution of sub-populations in sham and DVT groups. d GO analysis of Neutrophil-1 or Neutrophil-3 in comparison to the other two sub-populations. e Heatmap of the top 15 enriched genes in each sub-population in comparison to the other two sub-populations. f Volcano plot of DEGs in total neutrophils (DVT vs sham group). g Heatmap of the top 25 up- and downregulated genes in DVT compared to sham group. Up- and downregulated genes were separate by a red line.
