Fig. 2: Dispersed lysosomes reveal alterations in mTORC1 signaling. | Communications Biology

Fig. 2: Dispersed lysosomes reveal alterations in mTORC1 signaling.

From: Transcription factor EB regulates phosphatidylinositol-3-phosphate levels that control lysosome positioning in the bladder cancer model

Fig. 2

A Immunofluorescence staining of the lysosomal-associated membrane protein 1 (LAMP1, CD107a) and mTOR in MGHU3, RT112, KU19-19 and JMSU1. The zoom shows the merged image for both proteins in the white box. Scale bars equal 15 µm B Quantification of mTOR intensity on lysosomes normalized to total cellular mTOR approximately 50 cells for each cell line; ****p < 0.0001 in a Kruskal-Wallis test with Dunn’s test for multiple comparison, error bars are SEM. C Western blot analysis of phosphorylated p70-S6 Kinase 1 (P-p70-S6K1 Thr389) and total p70-S6K1 as well as GAPDH loading control in MGHU3, RT112, KU19-19 and JMSU1 cells and quantification of P-p70-S6K1 levels normalized to GAPDH from n = 7 experiments, error bars are SEM, molecular weight markers are in kDa. D Western blot analysis of phosphorylated 4EBP1 (P-4EBP1 Ser65) and total 4EBP1 as well as GAPDH loading control in MGHU3, RT112, KU19-19 and JMSU1 cells and quantification of P-4EBP1 levels normalized to GAPDH from n = 3 experiments, error bars are SEM, molecular weight markers are in kDa.

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