Fig. 4: DDX3X colocalises with large VP5 aggregates at the nuclear rim distinct from the viral replication compartments.
From: RNA helicase DDX3X modulates herpes simplex virus 1 nuclear egress
HeLa cells seeded on coverslips were either a mock-treated or b–d infected wild-type strain 17+ virus at an MOI of 5 for 9 hpi. Cells were fixed and labelled for a, b DDX3X (red) and VP5 (green) c DDX3X (red) and ICP4 (green) or d gB (red) and VP5 (green). All nuclei were stained with Hoechst 33342 (blue). Cells were imaged using dual colour high-resolution STED microscopy. Scale bar represents 5 µm. The images are representative of three independent experiments.
