Fig. 6: DDX3X and the large VP5 nuclear aggregates are C-capsids dependent.
From: RNA helicase DDX3X modulates herpes simplex virus 1 nuclear egress
a HeLa cells were mock-treated or infected at an MOI of 5 with the viral mutant ∆1-50 UL25 (only makes A and B nuclear capsids) or corresponding KOS wild-type virus. At 9 hpi, cells were fixed and processed for immunofluorescence (DDX3X: red; VP5: green; Hoechst 33342 stained nuclei: blue). Images were acquired on a conventional confocal microscope. The scale bar represents 5 µm. b Ten random images (~200 cells per condition) were used to quantify the different VP5 phenotypes. The percentages are means of three independent experiments along with their standard deviation (bilateral Student T-tests; ***p < 0.001).
