Fig. 1: The annotation of a gene containing sqr and pdo in B. licheniformis.
a The gene cluster encoding H2S oxidation in B. licheniformis MW3. The arrow on a black line indicated the genes in the chromosome. The gene names, related homologs, and gene IDs were given. The red arrow was used to indicate the primers designed for co-transcription analysis (Fig. 4a and Supplementary Fig. 4). The intergenic regions among the gene cluster were numbered with Arabic numbers in blue color from IR1 to IR10. b The phylogenetic tree of 16 S rDNA of 12 bacteria that have TCS genes within 3 loci of an sqr-pdo operon. The locations of the TCS genes, sqr, and pdo in the chromosome of the corresponding strains. The arrow indicated the genes, and the related protein IDs were given in the arrows. The percentages of protein identities of NreB and NreC of B. licheniformis DSM 13 with the corresponding proteins in other bacteria were shown under the genes. Sequence alignment was done by using ClustalW, and the trees were built by using MEGA X with the Maximum Likelihood method and Tamura-Nei model. c Phylogeny analysis of the PAS domains encoded in the sensor genes with known PAS domains. The branches were colored, and their functions were noted. Sequence alignment was done by using ClustalW, and the tree was built by using MEGA X with the Neighbor-Joining method.
