Fig. 5: The confirmation of the promoter between nreC and sqr.

a The schematic diagram of the sqr-pdo-R strain. b The sqr-pdo-R strain was cultured in the modified M9 medium with the induction of 200 μM sulfide or 200 μM elemental sulfur for 2 h. The resting cells were used to oxidize sulfide. Three parallel experiments were performed to obtain the averages and standard deviations. Data are presented as mean ± SD. One-way ANOVA was performed to calculate the p-values. Asterisks indicate statistically significant difference (***p < 0.001, ns = (p > 0.05)), and asterisks indicate statistical comparisons to the sqr-pdo-R at a given time point.