Fig. 3: Cellular diversity of cerebral organoids. | Communications Biology

Fig. 3: Cellular diversity of cerebral organoids.

From: Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids

Fig. 3

a A schematic illustration of cell diversity of cerebral organoids matured in dynamic systems. b Immunofluorescence staining of specific neuronal/glial/endothelial cell markers (NEUN/GFAP, MBP/CD11b, CD31/MAP2) at 120 days of organoids matured in 4 dynamic systems (scale bars = 100 μm for 10× and 20 μm for 25× magnification images, independent replicates = 3, Zeiss LSM 880). c WB analysis for neuronal proteins of cerebral organoids matured in a shaker (1,5), spinner (2,6), RCCS (3,7), and µ-platform (4,8) at day 60 and 120, respectively (independent replicates = 2). d FACS analysis of microglia and astrocytes in RCCS organoids on day 60 (independent replicates = 2). e Heatmap data of the qRT-PCR analysis of cerebral organoids matured in static, spinner, shaker, RCCS, and µ-platform on day 30, day 60, and day 120 in comparison to generated organoids on day 15 (independent replicates = 2).

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