Fig. 3: CRISPR/Cas9 mutagenesis of BdorOR7a-6 and BdorOR13a.
From: Two odorant receptors regulate 1-octen-3-ol induced oviposition behavior in the oriental fruit fly
a Rates of survival and mutagenesis after microinjection. b The target region of BdorOR7a-6. (i) Gene structure of BdorOR7a-6 based on the genome sequence. Exons are shown as gray boxes and introns as lines. The gRNA target in the first exon contains a 20-nt guide sequence and the adjacent CGG highlighted in green is the protospacer adjacent motif (PAM). (ii) The genotypes of G0 mutants were determined by TA cloning and Sanger sequencing (deletions are shown as dashes highlighted in yellow). The identifier assigned to the mosaic G0 flies is shown in the front of each sequence, and the number of the deleted nucleotides is shown after each sequence. (iii) Sanger sequencing traces for WT, BdorOR7a-6−/+, and BdorOR7a-6−/− flies. The black region is the gRNA target site. (iv) Predicted protein sequences of the BdorOR7a-6 WT and mutant (−4 bp) alleles. c The target region of BdorOR13a, with (i–iv) indicating the same details as provided for above the BdorOR7a-6 mutants.
