Fig. 3: Phagocytosis is induced in cDC2, cDC1, CD14dim CD16+ monocytes in response to eLNP stimulation.
Phagocytosis measured using fluorescent beads and multiparametric flow cytometry. PBMCs were incubated overnight with stimulus (15 μg/mL (total lipids, or ~7.5 μg/mL ionizable lipid) eLNP or plain medium) and with beads for a further 3 h. a FACS plot examples of phagocytosis assay as geometric mean fluorescent intensity (MFI) by flow cytometry. b Phagocytosis after 24 h eLNP stimulation (yellow) compared to that in unstimulated cells (grey) of PBMCS. n = 18 for each group. Each individual circle represents one individual subject, n = 18. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by or non-parametric Mann Whitney T-test.
