Table 1 The ten shRNAs identified by the Sens.AI screen that were selected for further validations and development.
Label | Strand | Gene | Position | Screen score | SARS-CoV+ | IC50 [pM] | RNAi trigger guide | siRNA sequence |
|---|---|---|---|---|---|---|---|---|
S1 | + | nsp3 | 6989 | 3.18 | No | 38.9 | TTAAAACACCTAAAGCAGCGGT | UUAAAACACCUAAAGCAGCGGUTT |
S2 | + | nsp4 | 9067 | 2.97 | No | 16 | TTCATAAGCAACAGAACCTTCT | UUCAUAAGCAACAGAACCUUCUTT |
S3 | + | Spike | 23646 | 2.87 | No | 10.7 | TAAGCAACTGAATTTTCTGCAC | UAAGCAACUGAAUUUUCUGCACTT |
S4 | + | Spike | 24610 | 2.24 | Yes | 16.6 | TTTAGTAGCAGCAAGATTAGCA | UUUAGUAGCAGCAAGAUUAGCATT |
S5 | + | ORF7A | 27611 | 2.77 | No | 27.9 | TTAGGTGAAACTGATCTGGCAC | UUAGGUGAAACUGAUCUGGCACTT |
S6 | + | RdRP | 15528 | 2.58 | No | 42.6 | TAAAAGTGCATTAACATTGGCC | UAAAAGUGCAUUAACAUUGGCCTT |
S7 | + | RdRP | 15819 | 1.82 | Yes | 259.7 | AAGGTCAGTCTCAGTCCAACAT | AAGGUCAGUCUCAGUCCAACAUTT |
S8 | - | Spike | 4464 | 1.79 | Yes | 8.6 | TACATTACACATAAACGAACTT | UACAUUACACAUAAACGAACUUTT |
S9 | + | N | 28969 | 2.23 | Yes | 1469.5 | TTGGCCTTGTTGTTGTTGGCCT | UUGGCCUUGUUGUUGUUGGCCUTT |
S10 | - | ORF7A | 2324 | 1.87 | Yes | 19.3 | TACGAGGGCAATTCACCATTTC | UACGAGGGCAAUUCACCAUUUCTT |
