Fig. 5: Relationships between prokaryotic amplicon sequence variants (ASVs), relevant environmental variables, and process rates.
N2O production rates from NH4+ oxidation and NO3− reduction are symbolized by black arrows. Pairwise correlation coefficients between ASVs and sample traits were calculated using a two-component sPLS regression model and are presented as a clustered heatmap. Taxa with correlations to nitrification rates >0.30 are indicated by solid black lines and taxa with correlations to N2O production from NO3− reduction >0.50 are indicated by dashed lines. Hierarchical clustering of variables was achieved using a complete Euclidean distance method. ASV subnetwork assignments determined through WGCNA are indicated by colored rectangles on the vertical axis dendrogram. Taxonomic labels correspond to the lowest level of classification determined for each ASV through alignment with the SILVA database.
