Fig. 3: KYP, SUVH5, SUVH6 and HDA6 synergistically regulate KNAT1 and KNAT2 by H3K9me2 and H3 deacetylation. | Communications Biology

Fig. 3: KYP, SUVH5, SUVH6 and HDA6 synergistically regulate KNAT1 and KNAT2 by H3K9me2 and H3 deacetylation.

From: Arabidopsis histone H3 lysine 9 methyltransferases KYP/SUVH5/6 are involved in leaf development by interacting with AS1-AS2 to repress KNAT1 and KNAT2

Fig. 3

a Expression of KNOX genes in hda6, kyp, had6/kyp, kyp/suvh5/6, and hda6/suvh4/5/6 mutants. Gene expression was analyzed by RT-qPCR. RNA was extracted from 10-day-old plants grown under LD conditions. UBQ10 was used as an internal control. Error bars indicate SD. **p < 0.01 by t test. At least three independent biological replicates were performed with similar results. b Schematic diagrams of KNAT1 and KNAT2 genomic sections. The promoter regions (X, Y, and P), first exon (S), and coding region (E) analyzed in ChIP-qPCR are indicated. c, d ChIP-qPCR analysis of H3K9me2(c) and H3Ac(d) levels on KNAT1 and KNAT2 in 10-day-old plants. The amounts of DNA after ChIP were quantified by qPCR. The values of relative enrichment were normalized by the value of total H3, and ACT2 was used as internal control. Data points represent average of three technical replicates. Error bars correspond to standard deviations from three biological replicates. *p < 0.05, **p < 0.005 (Student’s t test).

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