Fig. 4: Hepatic levels of histone acetylation are not altered by SB-204990.

a AML12 cells were cultured under standard conditions and were treated with indicated doses of acetate, citrate, and SB-204990 for 16 h. Glucose concentration in basal conditions is 17 mM, and supplemented is 42 mM. Acid histone isolations were performed, and histone acetylation levels were analyzed by western blot. Representative images of western blots and Ponceau staining are shown. n = 3. b Acid histone isolations were performed, and histone acetylation levels were analyzed by western blot in the livers of mice treated or not with SB-204990 for 15 weeks. Representative images of western blots and Ponceau staining are shown. n = 3 for STD. n = 7 for STD-SB. n = 6 for HFD. n = 6 for HFD-SB. c Quantification of histone acetylation levels of several residues is depicted. n = 3 for STD. n = 7 for STD-SB. n = 6 for HFD. n = 6 for HFD-SB. Student’s t-test. d Representative images of western blots of protein lysine acetylation are shown. Molecular weight markers are depicted on the left. n = 6 for STD. n = 7 for STD-SB. n = 5 for HFD. n = 5 for HFD-SB. e Densitometric quantification of hepatic protein lysine acetylation by western blot. n = 6 for STD. n = 7 for STD-SB. n = 5 for HFD. n = 5 for HFD-SB. Student’s t-test. f Total Ac-CoA levels in hepatic tissue. n = 5 for STD. n = 6 for STD-SB. n = 4 for HFD. n = 5 for HFD-SB. Two-way ANOVA. SB: SB-204990. STD: standard diet. HFD: high-fat diet. Ac-Lys: Acetylated lysine. r.u.: relative units. Ut: untreated. Data shown are the means ± SEM.*p < 0.05 STD-SB vs. STD. #p < 0.05 HFD-SB vs. HFD.