Fig. 5: Multiomic analysis of the effects of SB-204990 in the liver; a major role in longevity pathways. | Communications Biology

Fig. 5: Multiomic analysis of the effects of SB-204990 in the liver; a major role in longevity pathways.

From: Metabolic reprogramming by Acly inhibition using SB-204990 alters glucoregulation and modulates molecular mechanisms associated with aging

Fig. 5

a Multiomics analysis in liver samples using transcriptomics, proteomics, and GC/MS-based metabolomics. Significantly altered transcripts, proteins, and metabolites are shown in Venn diagrams. Upregulation (red), downregulation (blue), and reciprocal regulation (black). b Drop shape analysis indicating diet-independent significant modulation in transcripts, proteins, and metabolites altered by SB-204990 in STD and HFD. c The JPA from MetaboAnalyst 5.0 was used to generate plots indicating the p-value and impact of the top 20 pathways with the lowest p-value according to the analytical scheme shown in Fig. 7b. d The Upstream Regulators module of IPA was used to generate plots depicting the top 25 upstream regulators with the lowest p-value according to the analytical scheme shown in Fig. 7b. e Significantly altered transcripts, proteins, and metabolites are shown in Venn diagrams depicting the effects the HFD vs. STD-responsive transcriptomic, metabolomic, and proteomic responses in the liver of mice treated or not with SB-204990. Upregulation (red), downregulation (blue), and reciprocal regulation (black). f Diagram indicating significant transcriptomic, metabolomic, and proteomic responses promoted by HFD in the liver of mice treated or not with SB-204990. g JPA plots indicating the p-value and impact of the top 20 pathways with the lowest p-value of HFD vs. STD according to the analytical scheme shown in Fig. 7f. Specific pathways are highlighted in yellow. h JPA plots indicating the p-value and impact of the top 20 pathways with the lowest p-value of HFD-SB vs. STD-SB according to the analytical scheme shown in Fig. 7f. Specific pathways are highlighted in blue. i Upstream Regulators plots depicting the top 25 upstream regulators with the lowest p-value according to the analytical scheme shown in Fig. 7f. Specific regulators are highlighted in yellow. j Upstream Regulators plots depicting the top 25 upstream regulators with the lowest p-value according to the analytical scheme shown in Fig. 7f. Specific regulators are highlighted in blue. Transcriptomic analysis; n = 4 for STD. n = 4 for STD-SB. n = 3 for HFD. n = 3 for HFD-SB. iTRAQ proteomic analysis n = 4. Metabolomic GC/MS analysis; n = 5 for STD. n = 7 for STD-SB. n = 6 for HFD. n = 6 for HFD-SB. SB: SB-204990. STD: standard diet. HFD: high-fat diet.

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