Fig. 2: CD83 is predominantly expressed in the precursor exhausted T cells.
From: CD83 expression characterizes precursor exhausted T cell population
a Extraction of the candidate surface marker genes with predominant expression in a precursor exhausted T cell population compared with the terminally exhausted or non-exhausted memory T cells. b–d NSG mice were subcutaneously inoculated with A375-mesothelin or A375-CD19 and then treated with CAR-T cells. c Representative flow cytometry plots analyzing the expression of individual molecules in the tumor-infiltrating T cells and peripheral blood T cells. Gating threshold for each molecule was determined based on the plots of the fluorescence minus one (FMO) control samples. d, e The frequency of cells that express the indicated molecules in the CCR7+PD1+, CCR7-PD1+, and PD1− T cell population within the tumor (d), (n = 5 samples from different mice, repeated measures one-way ANOVA with multiple comparison test) and in the CCR7+/− peripheral blood T cells (e), (n = 3 different samples, two-tailed paired t-test). *P < 0.05, **P < 0.01. f CD83 expression in the CCR7+/− intratumoral CAR-T cells was analyzed in the additional four mice using the A375-mesothelin model (n = 4 samples from different mice, paired two-tailed t-test).
