Fig. 4: CD83 upregulation upon antigen stimulation is attenuated along with terminal T cell differentiation.
From: CD83 expression characterizes precursor exhausted T cell population
a, b CD19-targeting CAR-T cells were stimulated by NALM6 in vitro and analyzed for CD83 expression at the indicated time points. a Representative flow cytometry plots in the CD8+ CAR-T cell population. b The mean fluorescence intensity (MFI) of CD83 is shown (n = 3 independent cultures, ordinary one-way ANOVA with multiple comparison test). c CD19-targeting CAR-T cells were repeatedly stimulated by NALM6. d The frequency of CD45RA+/−CCR7+ memory T-cell population was analyzed at the indicated time points (n = 4 independent cultures, ordinary one-way ANOVA with multiple comparison test). e, f CD83 expression was analyzed in the CD8+ CAR-T cell population upon repeated NALM6 stimulation. The data shown are representative flow cytometry plots (e) and the calculated CD83 MFI (f), (n = 4 independent cultures, ordinary one-way ANOVA with multiple comparison test). g, h Cultured T cells were restimulated by K562-OKT3 or K562-OKT3/CD80 and analyzed for CD83 expression. Representative flow cytometry plots (g) and the CD83 MFI at days 1, 3, and 8 after restimulation are shown (h), (n = 4 independent cultures, unpaired two-tailed t-test at each time point). Horizontal lines indicate mean values.
