Fig. 7: TYRP1 expression is not dependent on the ATP7A copper transporter. | Communications Biology

Fig. 7: TYRP1 expression is not dependent on the ATP7A copper transporter.

From: Pigmentation and TYRP1 expression are mediated by zinc through the early secretory pathway-resident ZNT proteins

Fig. 7

a Immunoblot analysis shows that the expression of TYRP1, TYR, and TYRP2 in SK-ATP7A-KO cells was comparable with levels in WT SK-MEL-2 cells. b TYR activity was substantially decreased in SK-ATP7A-KO cells. L-DOPA oxidase activity (upper graph, n = 3) and tyrosinase zymography (lower panel) were performed. c, d Loss of TYRP1 expression in SK-Z5Z7-DKO cells (c) and loss of TYR activity in SK-ATP7A-KO cells (d) were not altered in SK-Z5Z7ATP7A-TKO cells. e Schematic representation of the chimeric constructs analyzed in f. f Domain exchange analysis between TYR and TYRP1. TYRP1-TYR chimera mutant showed the same property as that of TYRP1 expressed in SK-ATP7A-KO and SK-Z5Z7-DKO cells. In a, c, and f, β-galactosidase (β-gal) was used as a transfection control. In b and d, statistical significance was determined using the one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. **p < 0.01. Each experiment was performed at least three times, and representative results from independent experiments are shown.

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