Table 4 Antibodies for Western Blotting and ELISAs for Detection of TNF-alpha and IL6.
From: DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice
Antibody | Company | Catalog Number | Dilution | Species | Clonality |
|---|---|---|---|---|---|
DIAPH1 | BD Biosciences | 610849 | 1:1000 | Mouse | Monoclonal |
SREBP-1 | Santa Cruz | sc-13551 | 1:2000 | Mouse | Monoclonal |
SREBP-1 | Novus Biologicals | NB100-2215 | 1:1000 | Rabbit | Polyclonal |
SREBP-2 | Santa Cruz | sc-13552 | 1:2000 | Mouse | Monoclonal |
ChREBP | Novus Biologicals | NB400-135 | 1:2000 | Rabbit | Polyclonal |
ChREBP | Cell Signaling | 58069 S | 1:1000 | Rabbit | Polyclonal |
Lamin A/C | Cell Signaling | 2032 S | 1:2000 | Rabbit | Polyclonal |
AKT Ser473 | Cell Signaling | 9271 S | 1:2000 | Rabbit | Polyclonal |
AKT | Cell Signaling | 9272 S | 1:2000 | Rabbit | Polyclonal |
AMPKα Thr172 | Cell Signaling | 2535 T | 1:1000 | Rabbit | Polyclonal |
AMPKα | Cell Signaling | 5831 T | 1:2000 | Rabbit | Polyclonal |
mTOR Ser2448 | Cell Signaling | 5536 T | 1:2000 | Rabbit | Polyclonal |
mTOR | Cell Signaling | 2972 S | 1:2000 | Rabbit | Polyclonal |
S6 Ser240/244 | Cell Signaling | 2215 S | 1:2000 | Rabbit | Polyclonal |
S6 | Cell Signaling | 2317 S | 1:2000 | Rabbit | Polyclonal |
ROCK1 | Cell Signaling | 4035 T | 1:2000 | Rabbit | Polyclonal |
LIMK1 Thr508 | Abcam | Ab194798 | 1:1000 | Rabbit | Polyclonal |
LIMK1 | Cell Signaling | 3842 S | 1:2000 | Rabbit | Polyclonal |
SSH1 Ser978 | ECM Biosciences | SP3901 | 1:1000 | Rabbit | Polyclonal |
SSH1 | ECM Biosciences | SP1711 | 1:1000 | Rabbit | Polyclonal |
COFILIN Ser3 | Cell Signaling | 3313 T | 1:1000 | Rabbit | Polyclonal |
COFILIN | Cell Signaling | 5175 T | 1:2000 | Rabbit | Polyclonal |
C/EBPα | Cell Signaling | 8178 S | 1:2000 | Rabbit | Polyclonal |
GAPDH | Santa Cruz | sc-32233 | 1:5000 | Mouse | Monoclonal |
Tubulin | Millipore Sigma | T5168 | 1:25000 | Mouse | Monoclonal |
IRDye 680RD Goat anti-mouse | LI-COR | 925-68070 | 1:5000 | Goat | Polyclonal |
IRDye 800RD Goat anti-rabbit | LI-COR | 925-32211 | 1:5000 | Goat | Polyclonal |
IL6 ELISA | R&D Systems | M6000B | |||
TNF-alpha ELISA | R&D Systems | MTA00B |
