Fig. 3: GO and KEGG enrichment analyses of the fracture proteome reveal distinct differences in metabolic-, immune- and matrix-related processes between successful and compromised bone healing in rats.

a Down-regulated pathways and processes in compromised bone healing tissue are related to amino acid and carbohydrate metabolism and extracellular matrix at day 3 and day 7, while oxidative metabolism processes are downregulated at day 14. Cell contraction and calcium signaling proteins are downregulated at day 3 and day 14 in particular. Total protein number (n) as identified by GO and KEGG enrichment analyses are indicated for each timepoint. b Protein cluster associated with immune responses and extracellular matrix—receptor interactions have been found up-regulated in compromised healing for all time points, while cell cycle proteins were upregulated at day 3 and 7 and lysosomal proteins increased at day 3 and day 14. Total protein number (n) as identified by GO and KEGG enrichment analyses are indicated for each time-point.