Fig. 2: Expression profiling of Mir125b-2 and validation of Mir125b-2 knockout mice.
From: Mir125b-2 imprinted in human but not mouse brain regulates hippocampal function and circuit in mice
a Schematic diagram of chromosome locations of human MIR125b2 and mouse Mir125b-2. Levels of Mir125b-2 were measured in male postnatal day 28 (P28) mice with qPCR in various organs (b, n = 3), from the whole brain at distinct developmental stages (c, n = 9), and in multiple regions of the brain (d, n = 12). One-way ANOVA with Fisher LSD method post hoc comparison. e Schematic diagram of targeting locus of pre-miR-125b-2 in WT mice (top) and Mir125b-2 knockout mice (bottom). The bottom schematic indicates the pre-miR-125b-2 sequence was completely deleted. f Mir125b-2 knockout mice were genotyped by PCR with WT and KO allele primers shown in (e). g Schematic diagram of mouse mating strategy for generating paternal Mir125b-2 knockout (Mir125b-2m+/p−), maternal Mir125b-2 knockout (Mir125b-2m−/p+), and homozygous Mir125b-2 knockout (Mir125b-2m−/p−) offspring. WT = wild-type mice; HET = heterozygous knockout mice. h Expression levels of mature miR-125b-2 were measured with qPCR from the hippocampus of adult Mir125b-2m−/p− mice and their corresponding wild-type (Mir125b-2m+/p+) controls. Mann–Whitney rank-sum test. Mir125b-2m+/p+, n = 6 mice; Mir125b-2m−/p−, n = 4 mice. i Body weight and j brain weight were measured from male adult Mir125b-2m−/p− mice and their corresponding wild-type (Mir125b-2m+/p+) controls. Student’s t-test, two-tailed. Mir125b-2m+/p+, n = 12 mice; Mir125b-2m−/p−, n = 9 mice. Expression levels of Mir125b-2 were measured in the cerebral cortex at P28 with qPCR (k) from Mir125b-2m+/p− mice (blue bar), Mir125b-2m−/p+ mice (red bar), and corresponding controls (black bar). n = 11 mice for Mir125b-2m+/p− mice; n = 8 for Mir125b-2m−/p+ mice; n = 14 for controls. Mann–Whitney rank-sum test, #P < 0.1, *P < 0.05, **P < 0.01, ***P < 0.001. All data are presented as mean ± s.e.m. B: brain. E: eye. S: spleen. C: colon. H: heart. T: testis. L: lung. S#: stomach. K: kidney. A: adrenal gland. I: small intestine. L#: liver. Cx: cerebral cortex. T&H: thalamus and hypothalamus. ST: striatum. CB: cerebellum. BS: brainstem. OB: olfactory bulb. HP: hippocampus.
