Fig. 4: Hippocampal granule cells of Mir125b-2^m−/p− mice displayed increased neuronal excitability.

a Representative responses of hippocampal granule cells from wild-type (Mir125b-2^m+/p+) and Mir125b-2 homozygous knockout (Mir125b-2^m−/p−) mice to different current injections. Scale bar represents 10 mV and 100 ms. b Quantification of the frequency of action potential firing during the indicated magnitude of current injection. (Mir125b-2^m+/p+, n = 15 slices from six female mice; Mir125b-2^m−/p−, n = 17 slices from seven female mice). c Representative traces of recordings of first action potential from hippocampal granule cells of Mir125b-2^m+/p+ and Mir125b-2^m−/p− mice. Scale bar represents 10 mV and 100 ms. d Bar graphs show the input resistance, rheobase, resting membrane potential, membrane time constant from voltage response between 0 pA and 1-s hyperpolarizing current injection of −30 pA, and threshold potential (top); the maximum rise slope of first action potentials, the first action potential amplitudes, half-width, and amplitudes of fast and medium after-hyperpolarization potential (fAHP and mAHP) (bottom). Statistical significance was assessed using two-way repeated measured ANOVA with Holm–Sidak post hoc comparison (b), or Student’s t-test, two-tailed (d), *p < 0.05, **p < 0.01, ***p < 0.001. The significance for (d) disappeared after Bonferroni correction. All data are the mean ± s.e.m.