Fig. 5: Hippocampal granule cells of Mir125b-2m−/p− mice displayed impaired excitatory and inhibitory synaptic transmission.
From: Mir125b-2 imprinted in human but not mouse brain regulates hippocampal function and circuit in mice
Schematic of cell patching on adult hippocampal granule cells (GC) at postnatal day 49 (P49) for measurements of miniature excitatory postsynaptic current (mEPSC) (a) and miniature inhibitory postsynaptic current (mIPSC) (g). Representative traces of mEPSC (b) and mIPSC (h) recorded from wild-type (Mir125b-2m+/p+) and Mir125b-2m−/p− mice. Scale bar represents 20 pA and 30 s. Frequency (c) and cumulative probability of inter-event interval (d) are shown for mEPSC. Amplitude (e) and its cumulative probability (f) are shown for mEPSC. Frequency (i) and cumulative probability of inter-event interval (j) are shown for mIPSC. Amplitude (k) and its cumulative probability (l) are shown for mIPSC. mEPSC: Mir125b-2m+/p+, 14 cells, 3 mice; Mir125b-2m−/p−, 19 cells, 4 mice. mIPSC: Mir125b-2m+/p+, 19 cells, 4 mice; Mir125b-2m−/p−, 18 cells, 4 mice. Student’s t-test, two-tailed, *P < 0.05, **P < 0.01, ***P < 0.001. All data are presented as mean ± s.e.m.
