Fig. 2: LncAABR07053481 can inhibit hypoxia-induced apoptosis of BMSCs. | Communications Biology

Fig. 2: LncAABR07053481 can inhibit hypoxia-induced apoptosis of BMSCs.

From: LncAABR07053481 inhibits bone marrow mesenchymal stem cell apoptosis and promotes repair following steroid-induced avascular necrosis

Fig. 2

a The expression of LncAABR07053481 was detected by qPCR (n = 4). b–e Detection of BMSC apoptosis under different conditions by Annexin V-fluorescein isothiocyanate (FITC, green)/propidium iodide (PI, red) (n = 4). f–i The expression levels of CASP-3, Survivin, and Bcl-2 were detected by western blotting (n = 3). j Forty-eight hours after surgery, the fluorescence intensity of 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR, red) in the transplantation area was detected by live imaging of small animals (n = 7). k Quantitative analysis of DiR fluorescence intensity in the transplanted area (n = 7). l–m At 48 h after surgery, TdT-mediated dUTP nick-end labeling (TUNEL, red) /4’,6-diamidino-2-phenylindole (DAPI, blue) was used to detect apoptosis in the transplantation area (n = 7). Data were shown as mean  ±  S.D. *P < 0.05; in (a, c, e, g–i, k, m), statistical significance was calculated by one-way ANOVA with Tukey’s post hoc test.

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