Fig. 3: LncAABR07053481 inhibits hypoxia-induced apoptosis of BMSCs by regulating the Notch1 pathway. | Communications Biology

Fig. 3: LncAABR07053481 inhibits hypoxia-induced apoptosis of BMSCs by regulating the Notch1 pathway.

From: LncAABR07053481 inhibits bone marrow mesenchymal stem cell apoptosis and promotes repair following steroid-induced avascular necrosis

Fig. 3

a mRNA clustering analysis (n = 3). b Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed genes. c Gene set enrichment analysis (GSEA). d The expression of Notch1 was detected by qPCR (n = 3). e–g The expression levels of Notch1 and NICD1 were detected by western blotting (n = 3). h, i Detection of BMSC apoptosis by Annexin V-FITC/PI (n = 3). j–n The expression levels of Notch1, NICD1, Survivin, and Bcl-2 were detected by western blotting (n = 3). Data were shown as mean  ±  SD. *P < 0.05; In (d, f, g), statistical significance was calculated by Student’s t-test; In (i, k–n), statistical significance was calculated by one-way ANOVA with Tukey’s post hoc test.

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