Fig. 4: LncAABR07053481 functions as a ceRNA and sponges miR-664-2-5p in BMSCs. | Communications Biology

Fig. 4: LncAABR07053481 functions as a ceRNA and sponges miR-664-2-5p in BMSCs.

From: LncAABR07053481 inhibits bone marrow mesenchymal stem cell apoptosis and promotes repair following steroid-induced avascular necrosis

Fig. 4

a RNA fluorescence in situ hybridization (RNA-FISH) detection of the subcellular localization of LncAABR07053481, 18S, and U6 were used as positive control. (n = 4). b Bioinformatics tools predict the miRNAs that may bind to the untranslated region at the 3’ end of Notch1 mRNA (Notch1 mRNA 3′UTR) and LncAABR07053481. c Binding sites of miR-664-2-5p with LncAABR07053481 and Notch1 mRNA 3′UTR. d, e Luciferase reporter assay shows that miR-664-2-5p binds to LncAABR07053481 and Notch1 mRNA (n = 5). f–h RNA immunoprecipitation (RIP) detection of LncAABR07053481 enrichment to miRNA ribonucleoprotein complexes (miRNPs) (n = 4). g–i RIP detection of enrichment of Notch1 mRNA to miRNPs (n = 4). Data were shown as mean  ±  SD. *P < 0.05; In (d–i), statistical significance was calculated by one-way ANOVA with Tukey’s post hoc test.

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