Fig. 7: Effect and mechanism of LncAABR07053481 on the repair of early SANFH by regulating the Notch1 pathway to inhibit hypoxia-induced apoptosis of BMSCs: LncAABR07053481 acts as a sponge for miR-664-2-5p to attenuate its repressive effect on Notch1 and activates the Notch1 pathway and releases NICD1 (the intracellular active fragment of Notch1) from the cell membrane.

NICD1 localizes to the nucleus, where it forms a transcriptionally active complex with the DNA-binding protein CSL and the coactivator Mastermind (MAM) to upregulate transcription of Notch target genes (Survivin and Bcl-2). Following which, hypoxia-induced apoptosis of BMSCs in the area of osteonecrosis is inhibited, improving the transplantation effect of BMSCs on early SANFH.