Fig. 1: Workflow for human plasma transferrin (Tf) isolation and N-glycan structures characterization.
From: Comparative analysis of transferrin and IgG N-glycosylation in two human populations
a Sequential isolation and N-glycan profiling of immunoglobulin G (IgG) and Tf from two human populations (Korcula and VIKING). HILIC-UHPLC-FLD ultra-high-performance liquid chromatography based on hydrophilic interactions with fluorescent detection. b Workflow for Tf purity assessment and N-glycan structures characterization by several complementary approaches. nanoLC-ESI-qTOF-MS nano-liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry; MALDI-TOF-MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
