Fig. 3: Phenotypic validation of the candidate gene.
a The proliferation rates of WT, Cont (cells infected with control sgRNA) and target gene knockout cells. The indicated cells (1000 cells per cm2 in 12-well plates) were cultured in L/S for four days, and the cell numbers were counted. b Colony numbers of the indicated cells. Cells were seeded into 12-well plates at a density of 200 cells per well and grown for 6 days, and colony numbers were counted. c Morphology of colonies formed by the indicated cells. Scale bar, 200 μm. d Colonies were fixed and stained for AP and then scored as undifferentiated, mixed, or differentiated. Data in (a, b and d) are represented as the mean ± SD; n = 3.
