Fig. 2: Affinity heatmaps of coronatine stereoisomers on COI1-JAZ co-receptors of A. thaliana and S. lycopersicum. | Communications Biology

Fig. 2: Affinity heatmaps of coronatine stereoisomers on COI1-JAZ co-receptors of A. thaliana and S. lycopersicum.

From: Subtype-selective agonists of plant hormone co-receptor COI1-JAZs identified from the stereoisomers of coronatine

Fig. 2: Affinity heatmaps of coronatine stereoisomers on COI1-JAZ co-receptors of A. thaliana and S. lycopersicum.

a Heatmap analyses of Kd value of all stereoisomers for functional AtCOI1-AtJAZ pairs. White means “+not determined” due to a low increase of FA (Supplementary Fig. 4). The color bar represents the Kd value of each stereoisomer. Kd value of each stereoisomer which increased FA value at 1 µM was calculated by dose-response. b Evaluation of GUS activity in the roots of 4-day-old 35S: JAZ1-GUS, 35S-JAZ9-GUS, and 35S: JAZ10-GUS seedlings. Images of each seedling stained with 5-bromo-4-chloro-3-indolyl glucuronide were pretreated for 2 h with or without ligand (1 μM). Similar results were obtained in three independent experiments. Scale bar, 1 mm. c Heatmap analyses of Kd values of all stereoisomers for SlCOI1-SlJAZ pairs. White means “not determined” due to a low increase of FA (Supplementary Fig. 10). The color bar represents the Kd value of each stereoisomer. Kd value of each stereoisomer which increased FA value at 1 µM was calculated by dose-response curves.

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