Fig. 4: Gene expression analyses of 2- and 2e-treated Col-0 and jaz9-1 seedlings.

a Principal component analysis of upregulated genes in each compound-treated 6-day-old Arabidopsis seedlings by RNAseq analyses (fold change >2.5, FDR < 0.1). Black, blue, red, and green dots showed three independent replicates of each treatment. b Venn diagram (FDR < 0.1) indicates the number of upregulated genes at least 2.5-fold change in response to different treatments. c Heatmap illustrating changes in gene expression of jasmonate response to different treatments (FDR < 0.1). Each GO ID is as follows: Regulation of JA mediated signaling pathway: 2000022, Response to JA: 0009753, Defense response: 0006952, Metabolic process: 0008152. d Venn diagram (FDR < 0.1) indicating the number of downregulated genes at least 2.5-fold change in response to different treatments and GO enrichment analysis. e Gene expression analyses of cell wall organization-related genes by RT-qPCR in Col-0 and coi1-1 seedlings (n = 4) with or without compound treatment (1 µM) for 8 h. Similar results were obtained in three independent experiments. Significant differences were evaluated by one-way ANOVA/Tukey HSD post hoc test (p < 0.05). Values are mean ± SE. f Venn diagram (FDR < 0.1) indicating the number of upregulated genes at least 2.5-fold change in response to different treatments in Col-0 and jaz9-1. g RT-qPCR analyses in Col-0 and jaz9-1 seedling (n = 4) with or without compound treatment (1 µM). Each relative expression fold was calculated using an average expression level in Mock- or 2e-treated Col-0 or jaz9-1 seedlings. Similar results were obtained in three independent experiments. Significant differences were evaluated by Student’s t-test (**p < 0.01, *p < 0.05; ns, not significant). Values are mean ± SE.