Fig. 1: Metagenomic phage display library. | Communications Biology

Fig. 1: Metagenomic phage display library.

From: New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota

Fig. 1

a Schematic representation of metagenomic phage display library preparation and biopanning to screen ligand binding recombinant phages. b Agarose gel electrophoresis of PCR amplicons indicating the presence or absence of any recombinant insert within randomly selected phages of the metagenomic phage display library. Results of a single screening experiment. Lanes 1 and 21: 1 kbp DNA marker (NEB). Lanes 20 and 34: 100 bp DNA marker (NEB). Lanes 2–19 and 22–33: PCR amplicons of the phages.

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