Fig. 2: Identification of mucin binding protein domains. | Communications Biology

Fig. 2: Identification of mucin binding protein domains.

From: New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota

Fig. 2

a–f Agarose gel electrophoresis of PCR amplicons indicating the presence or absence of any recombinant insert following biopanning of the metagenomic phage display library against porcine stomach type II mucin. Results of a single screening experiment. a Lane 1: 1 kbp DNA marker. Lanes 2–9: Phages eluted with L-fucose, b Lane 1: 1 kbp DNA marker. Lanes 2–9: Phages eluted with D-galactose, c Lane 1: 1 kbp DNA marker. Lanes 2–9: Phages eluted with GalNAc, d Lane 1: 1 kbp DNA marker. Lanes 2–9: Phages eluted with GlcNAc, e Lane 1: 1 kbp DNA marker. Lanes 2–9: Phages eluted with D-mannose, f Lane 1: 1 kbp DNA marker. Lanes 2–9: Phages eluted with sialic acid (Neu5Ac), g Coomassie blue stained SDS-PAGE showing purified MG1 and MN3 proteins in the imidazole eluates following Ni-NTA metal ion affinity chromatography; Lane 1: Purified MG1 protein; Lane 2: Protein marker from NEB (2-212 kDa); Lane 3: Purified MN3 protein, h Coomassie blue stained SDS-PAGE showing purified MU1 protein in the imidazole eluates following Ni-NTA metal ion affinity chromatography; Lane 1: Protein marker from NEB (2–212 kDa); Lane 2: Purified MU1 protein, i Coomassie blue stained SDS-PAGE showing purified MU3 protein in the imidazole eluates following Ni-NTA metal ion affinity chromatography; Lane 1: Protein marker from NEB (2–212 kDa); Lane 2: Purified MU3 protein. j–n AlphaFold2 predicted structures of MG1 (j), MN3 (k), MU1 (l), MU3 (m), MF12 (n), and MF12F2 (o).

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