Table 1 Ligand binding profile of clones (MG1, MN3, MU1, and MU3) identified by biopanning against mucin

Glycan Array (CFG glycan array version 5.3 comprising 600 natural and synthetic glycans)^a

Asialo, long, complex N-glycans; terminal α1,2-Fuc

Asialo, short, complex N-glycans; terminal LacNAc; H, Le^x, Le^y antigens

Asialo, long, complex N-glycans; terminal LacNAc; H, Le^x, Le^y antigens

Sialo, short, complex N-glycans; terminal α2,6-Sia

ITC (LNnT, LNT, H type-2 pentaose, Fetuin, Asialofetuin, H type2 heptaose, Le^a, difucohexaose, Le^b, Le^x, H type-2 triose^b, LNnO^c, LNnH^c, A type-2 tetraose^c)

None

H type-2 pentaose

H type-2 pentaose

Fetuin

ELLA (PAA-Biotin and PAA-Biotin-linked α-D-Mannose, β-D-Mannose, α-Neu5Ac, α-L-Fucose, β-D-GlcNAc, α-D-Galactose, β-D-Galactose, Le^y, H type-2 triose)

Not tested

PAA-Biotin-Le^y, PAA-Biotin-H type-2 triose

Not tested

Not tested