Fig. 2: Brain regions activated by song in 55-day-old birds.
From: Tracing development of song memory with fMRI in zebra finches after a second tutoring experience
a, b Statistical map of voxels activated by song superimposed on coronal and corresponding (indicated with cross hair) parasagittal sections of the high-resolution atlas of the zebra finch brain84. Only voxels with t > 1.76 (one-sample t tests, exploratory threshold Puncorrected < 0.05) are displayed (A: TUT1 > rest; B: NOV > rest); t-values are color-coded based on the scale on the right. c, d Paired t tests show a significantly higher BOLD response in forebrain regions, including lMAN, in response to novel song (NOV vs. TUT1 playback). All voxels with t > 2.47 are displayed (paired t tests, Puncorrected < 0.01) and t-values are color-coded based on the scale displayed in the figure. e Line drawing of a parasagittal section from the zebra finch histological atlas90. f Region-of-interest (ROI) analysis restricted to lMAN shows a higher BOLD response to NOV vs. TUT1 in the right lMAN. g Boxplot of BOLD response (β weights) for each stimulus relative to rest periods within lMAN (indicated with a white arrow in (f)). Boxplots showing the interquartile range (box), median (black line), and 1st and 3rd quartile; each circle represents data from one individual bird (*PFWE < 0.05, N = 28). For visual representation, one data point (bold estimate = −9.50416, left hemisphere, NOV) was excluded from the figure but not from the statistical analysis. h, i Detailed views of the exact location of the activated cluster observed within lMAN. NCL: caudolateral Nidopallium, CMM: caudomedial Mesopallium, lMANco: core of the lateral magnocellular nucleus of the anterior Nidopallium, L2a: subfield L2a of field L. HVC and Field L are used as proper names.
