Fig. 3: BiTE mediated activation of Jurkat-ZsG reporter cells in droplets.
From: A high throughput bispecific antibody discovery pipeline
a Illustrative mechanism of CD19xCD3 BiTE mediated T cell activation in the droplet assay. R.E. responsive element. b Microscopic images of activated Jurkat-ZsG reporter cells (expressing a GFP reporter) in the presence of Raji cell (CD19+) and a research-grade recombinant Blinatumomab in droplets; scale bar, 100 µm. Green, ZsGreen reporter signal; Red, a cell tracking dye pre-labeled for Raji. c ZsGreen signal intensity change in Jurkat-ZsG cells added with 100 ng/ml Blinatumomab, without Raji cells (i) and with Raji cells (ii) over 0–9 h time-points post-incubation. ZsGreen intensity was quantified for randomly chosen droplets (obtained from one encapsulation experiment) at each time point. Data for each timepoint is presented as a scatterplot with \({{{{{\rm{mean}}}}}\,\pm {SD}}\) error bars.
