Fig. 6: Characterization of activity of purified Clone 5 and Clone 6 with primary T cells.
From: A high throughput bispecific antibody discovery pipeline
a Activation profile of CD4 + T cells (assessed by the percentage of double-positive CD69+ CD25+ population) in the presence of Raji cells and purified BiTE clones at varying concentrations. b Percentage of PD-1 expressing CD4+ T cells in the presence of Raji cells and purified BiTE clones at varying concentrations. c Activation profile of CD8+ T cells (assessed by the percentage of double-positive CD69+ CD25+ population) in the presence of Raji cells and purified BiTE clones at varying concentrations. d Percentage PD-1 expressing CD8+ T cells in the presence of Raji cells and purified BiTE clones at varying concentrations. e Apoptosis of Raji cells in the presence of pan T cells (E:T = 10:1), isolated from donor PBMCs and purified BiTE clones at different concentrations assessed by Annexin V/7AAD staining of Raji cells. Data are presented as XY plots, and each data point is obtained as a mean of \(n=3\) replicates (in the case of Clone 5 and Clone 6) for pan T cells isolated from \(n=1\) donor PBMCs. \({{{{{\rm{Mean}}}}}\pm {SD}}\) error bars are plotted for each data point. In the case of Blinatumomab, data is obtained from \(n=2\) replicates, and error bars are not shown.
