Fig. 7: Distribution of design parameters in the functionally active BiTE clones sorted using the single cell discovery platform. | Communications Biology

Fig. 7: Distribution of design parameters in the functionally active BiTE clones sorted using the single cell discovery platform.

From: A high throughput bispecific antibody discovery pipeline

Fig. 7: Distribution of design parameters in the functionally active BiTE clones sorted using the single cell discovery platform.The alternative text for this image may have been generated using AI.

a Distribution of relative orientation of CD19 and CD3 scFvs (i), Distribution of CD3 scFvs (ii), Distribution of CD19 scFvs (iii), and linkers (iv) in the functionally active sorted library. The sequence of 99 functional BiTE clones (98 unique clones+1 Blinatumomab sequence) elucidated by sanger sequencing was used for analyzing the distribution of design parameters. b CDRH3 composition of sorted BiTEs having a 4G7 (i), B43(ii), JUNO241 (iii) or FMC63 (iv) aCD19 scFv. CDRH3 compositions for each CD19 scFv type were analyzed from n = 34 4G7 containing BiTEs, n = 25 B43 containing BiTEs, n = 29 JUNO241 containing BiTEs, and n = 8 FMC63 containing BiTEs. Three clones were excluded from the CDRH3 analysis due to sequence ambiguity in the CDRH3 region.

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