Fig. 3: The interaction between UBAP2L and G3BP1 is required for SG assembly.

a List of proteins identified by mass spectrometry analysis. b 293T cells were transfected with FLAG-UBAP2L together with GFP-G3BP1 or GFP-FXR2. After 24 h, the cells were lysed and immunoprecipitated with an anti-FLAG antibody. The immunoprecipitates were immunoblotted for GFP and FLAG. c HeLa cells were lysed and immunoprecipitated with either anti-rabbit IgG or anti-UBAP2L antibodies. The immunoprecipitates were blotted with anti-UBAP2L, anti-G3BP1 or anti-FXR2 antibodies. d Schematic representation of UBAP2L deletion constructs (N.D. (not detected)). e HeLa cells that constitutively expressed each construct were treated with 0.5 mM arsenite for 30 min and immunostained for TIAR and FLAG (scale bar = 10 μm). f HeLa cells that constitutively expressed each siRNA resistant cDNA were depleted of endogenous UBAP2L by UBAP2L siRNA #2. The cells were lysed and immunoblotted with anti-FLAG and anti-UBAP2L antibodies. g Cells that constitutively expressed each constructs were depleted of UBAP2L by siRNA and immunostained for FLAG and G3BP1 (scale bar = 10 μm). h The number of G3BP1-positive SGs per cell is presented in the graphs (n = 3 (total cells = 15), **P < 0.01, *P < 0.05). i The number of TIAR-positive SGs per cell is presented in the graphs (n = 3 (total cells = 15), **P < 0.01, *P < 0.05).