Fig. 5: C/D box snoRNAs mediate the association between UBAP2L and G3BP1.

a The small RNAs with the top 10 UBAP2L RPM scores are presented (n = 2). b The percentages of each type of small RNAs with the top 100 UBAP2L RPM scores are shown in the circle graph (n = 2). c Small RNAs were isolated from immunoprecipitates from the lysate of 293T cells that constitutively expressed the Halo tag, Halo-UBAP2L, Halo-G3BP1, or Halo-CPEB. The levels of SNORD44 and SNORD49A were examined by quantitative RT-PCR (qRT-PCR) (n = 3, **P < 0.01, N.S. (not significant) P > 0.05). d Immunoprecipitated FLAG-UBAP2L was mixed with recombinant GST or GST-G3BP1 and incubated with or without in vitro-transcribed SNORD44 sense (S) or anti-sense (AS) sequences. Protein-RNA complex precipitated by glutathione agarose beads were immunoblotted with an anti-FLAG antibody. CBB indicates Coomassie brilliant blue staining of recombinant proteins. The number in the middle of the figure and the graphs indicates the quantitative value measured with ImageJ. e In vitro-transcribed SNORD30, SNORD49A, or SNORD56 were mixed with immunoprecipitated FLAG-UBAP2L and GST-G3BP1 and subjected to precipitation with glutathione agarose beads followed by immunoblot analysis. Both UBAP2L and G3BP1 RPM scores of SNORD56 is significantly lower than that of SNORD30 and SNORD49A. f SG core fraction was purified from arsenate treated HeLa cells and no-treated HeLa cells. RNA was purified each fraction. The levels of SNORD44 and SNORD49A were examined by qRT-PCR (n = 3, *P < 0.05).