Fig. 2: Isolation of a GA receptor agonist, diphegaractin, and its specific activity for VvGID1 and VvDELLA proteins. | Communications Biology

Fig. 2: Isolation of a GA receptor agonist, diphegaractin, and its specific activity for VvGID1 and VvDELLA proteins.

From: Identification of a new gibberellin receptor agonist, diphegaractin, by a cell-free chemical screening system

Fig. 2

a Result of GA receptor agonist screening using a chemical library containing 9600 chemical compounds. b Flow chart of GA receptor agonist isolation. c Dose-dependent activity of diphegaractin for interaction between VvGID1a and VvDELLA proteins. The interactions of VvGID1a with VvDELLA proteins were analysed by AlphaScreen system using GA3 or diphegaractin at various concentrations. Data are shown as mean and individual data points from three independent experiments. d Dose-dependent activity of diphegaractin for interaction between VvGID1b and VvDELLA proteins. The interactions of VvGID1b with VvDELLA proteins were analysed by AlphaScreen system using GA3 or diphegaractin at various concentrations. Data are shown as mean and individual data points from three independent experiments.

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