Fig. 5: Subunit arrangement in the sweet potato VLPs. | Communications Biology

Fig. 5: Subunit arrangement in the sweet potato VLPs.

From: CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host

Fig. 5: Subunit arrangement in the sweet potato VLPs.

a CryoEM map of SPFMV coloured according to the subunit elevation along a helical turn (starting subunit in cyan and 10th subunit in dark blue). Right panel shows the corresponding structural model of one complete segment with first, second and 10th subunits labelled. b CryoEM map of SPFMV (top panel) coloured according to N-termini of first CP (in purple), N + 1 (in cyan) and N + 10 CP (in dark blue) along the helical segment. The three CPs are coloured in pink with the C-terminus in orange. Bottom panel shows cryoEM map of SPMMV, coloured according to N-termini of first CP (in gold), N + 1 (in cyan) and N + 10 (in dark blue). Middle panels show a close-up view of the N-terminus organization between Nth, N + 1 and N + 10 CP in both SPFMV and SPMMV structures. Right panels show the outlined, zoomed-in area for N-terminal interaction with Nth CP via N + 1 (cyan) and N + 10 (dark blue) N-terminus in SPFMV (top right) and SPMMV (bottom right) respectively. Key residues are labelled with the Nth CP shown as gaussian filtered map to illustrate binding pockets, N + 1 model in pink (SPFMV) and in orange (SPMMV), with their N-terminal segments in cyan and the N + 10 N-terminal segments shown in dark blue.

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