Fig. 3: Expression of origin-specific markers in DE-MSCs.
From: Definitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells
a Schematic flow of in vitro MSC generation from mesoderm (Meso-MSCs), neural crest (NC-MSCs), and trophoblast (Troph-MSCs) origins, scale bar = 400 μm. b Heatmap of DEG in NC-MSCs, Troph-MSCs, Meso-MSCs, DE-MSCs (CHIR/SB), DE-MSCs (CHIR), and DE-MSCs (FBS) compared with hESCs and cell type prediction by Enrichr database, indicating that all these MSC highly expressed the genes related to fibroblast rather than hESCs. c Heatmap of DEG in DE-MSCs (CHIR/SB), DE-MSCs (CHIR), DE-MSCs (FBS), Meso-MSCs, NC-MSCs, and Troph-MSCs and cell type prediction by Enrichr database, showing that MSCs originated from different developmental origin has organ-specific gene expression signatures. d Heatmap of DEG in DE-MSCs (CHIR/SB), DE-MSCs (FBS), and DE-MSCs (CHIR) and cell type prediction by Enrichr database, indicating CHIR or CHIR/SB treatment from day 3 to day 5 modified the gene signatures of DE-MSCs.
